Related Experiment Video
Updated: Aug 14, 2025
09:11
Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion
Published on: September 25, 2019
7.7K
Expansion microscopy: A revolution in diagnostic pathology.
Snehashish Ghosh1, Bhawana Subedi Sapkota2, Roopa S Rao3
1Department of Oral Pathology, College of Medical Sciences, Bharatpur, Nepal.
Journal of Microscopy
|January 15, 2023
Summary
Expansion microscopy (ExM) offers physical magnification for improved diagnostic pathology and microbiology. This technique enhances specimen resolution and enables nanoscale imaging, yielding brilliant results in various tissue and microbiological samples.
Area of Science:
- Pathology
- Microbiology
- Microscopy
Background:
- Optical microscopy is a long-standing technique in histopathology and microbiology.
- Its simplicity, specimen compatibility, and ease of use are widely accepted by pathologists.
Purpose of the Study:
- To introduce Expansion Microscopy (ExM) as a novel diagnostic tool.
- To highlight ExM's advantages over traditional optical microscopy.
Main Methods:
- Utilizing physical magnification of tissue specimens.
- Employing ExM and its variants for sample analysis.
Main Results:
- ExM significantly improves specimen resolution compared to optical methods.
- ExM enhances diagnostic precision and allows for nanoscale imaging.
- Successful application of ExM in diverse tissue and microbiological specimens.
Conclusions:
- Expansion microscopy represents a significant advancement in diagnostic pathology and microbiology.
- ExM provides superior resolution and diagnostic capabilities for detailed specimen analysis.
Related Concept Videos
01:28Three-Dimensional Microscopy in Microbiology
149
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
149
01:22Overview of Microscopy Techniques
10.6K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
10.6K
01:29Two-Dimensional Microscopy in Microbiology
195
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
195
01:37Super-resolution Fluorescence Microscopy
7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
01:16Confocal Fluorescence Microscopy
13.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.5K