Electrokinetic Extraction of Doxorubicin from Biological Fluids by Polymer Inclusion Membrane Sampling Probe.

Area of Science:

• Analytical Chemistry
• Materials Science
• Biomedical Engineering

Background:

• Electrokinetic extraction offers a promising technique for analyzing drugs in complex biological matrices.
• Developing efficient and sensitive sampling probes is crucial for accurate drug quantification.
• Polymer inclusion membranes (PIMs) present a versatile platform for selective analyte extraction.

Purpose of the Study:

• To develop and characterize a PIM-based sampling probe for electrokinetic extraction of drugs.
• To evaluate the probe's efficiency in extracting doxorubicin from various biological fluids.
• To assess the integration of the probe into a portable device for practical applications.

Main Methods:

• Fabrication of a PIM-based probe via dip-coating a glass capillary with a PIM solution (CTA:NPOE:[EMIM][NTf2]).
• Electrokinetic extraction of doxorubicin from human plasma, serum, and dried blood spots (DBS).
• Quantification of extracted doxorubicin using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS).

Main Results:

• The PIM probe successfully electrokinetically extracted doxorubicin from plasma, serum, and DBS.
• Optimized conditions yielded a low quantification limit of 0.2-2 ng/mL for doxorubicin.
• The probe was integrated into a portable, low-voltage (36 V) device for field applications.

Conclusions:

• The developed PIM-based probe provides an efficient and sensitive method for electrokinetic drug extraction.
• The portable device facilitates safe and low-voltage electrokinetic extraction, enhancing analytical workflows.
• This technology holds potential for improved drug analysis in clinical and forensic settings.