Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Gene Regulation During Sporulation

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...

Stringent Response in E. coli

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

Transcription Elongation Factors

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...

Endospores and Sporulation

Endospores and Sporulation

Endospores are specialized, dormant cells primarily formed by Gram-positive bacteria, including Bacillus and Clostridium, enabling survival under extreme environmental conditions. Due to their unique composition and formation process, these structures are highly resistant to physical and chemical insults, such as extreme heat, ultraviolet and ionizing radiation, desiccation, and toxic chemicals. Rare instances of endospore-like structures have also been observed in some Gram-negative bacteria,...

Mechanism of Filopodia Formation

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...

Yeast Signaling

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Neutrophils repurpose the nucleolus as a cytokine reservoir and secretory organelle.

bioRxiv : the preprint server for biology·2026

Same author

SNOR promotes translation restart after dormancy.

Nature·2026

Same author

Mechanism of nucleolytic degradation of human ribosomes.

bioRxiv : the preprint server for biology·2026

Same author

A Novel Eukaryotic Ribosome Factor Enables Translation Restart Following Cellular Dormancy.

bioRxiv : the preprint server for biology·2026

Same author

Starvation-independent alarmone production inhibits translation through GTP depletion.

bioRxiv : the preprint server for biology·2026

Same author

<i>Paenibacillus</i> encodes a membrane-localized Spo0B.

Journal of bacteriology·2025

Search research articles

Related Experiment Video

Updated: Aug 14, 2025

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

Published on: September 17, 2016

Elongation Factor P Is Important for Sporulation Initiation.

Heather A Feaga^1,2, Hye-Rim Hong¹, Cassidy R Prince¹

¹Department of Microbiology, Cornell University, Ithaca, New York, USA.

Journal of Bacteriology

|January 18, 2023

Summary

This summary is machine-generated.

Protein elongation factor P (EF-P) is crucial for bacterial translation. Its absence in Bacillus subtilis delays sporulation initiation by reducing expression of the master regulator Spo0A, indicating translation stress impacts this process.

Keywords:

EF-P elongation factor P protein synthesis ribosome quality control sporulation translation

More Related Videos

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

Published on: September 12, 2018

Microscopy of Fission Yeast Sexual Lifecycle

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

Related Experiment Videos

Last Updated: Aug 14, 2025

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

Published on: September 17, 2016

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

Published on: September 12, 2018

Microscopy of Fission Yeast Sexual Lifecycle

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

Area of Science:

Microbiology
Molecular Biology
Bacterial Physiology

Background:

The universally conserved protein elongation factor P (EF-P) aids translation, particularly at difficult peptide bonds like polyproline.
EF-P is not essential in most bacteria, and its precise physiological functions remain largely unknown.

Purpose of the Study:

To investigate the role of EF-P in Bacillus subtilis sporulation initiation.
To elucidate the molecular mechanisms underlying EF-P's influence on this developmental process.

Main Methods:

Comparative analysis of sporulation-specific gene expression in wild-type and Δefp strains.
Ribosome profiling to assess gene expression levels, specifically for the spo0A gene.
Complementation studies involving ectopic expression of Spo0A.

Main Results:

Deletion of EF-P (Δefp) in Bacillus subtilis significantly delays the initiation of sporulation.
Ribosome profiling revealed lower expression of spo0A, the master regulator of sporulation, in the Δefp strain.
Ectopic expression of Spo0A rescued the sporulation defect, confirming reduced spo0A expression as the cause.

Conclusions:

EF-P absence impairs Bacillus subtilis sporulation initiation by hindering Spo0A expression.
This study suggests that cells can detect translation stress and link it to the decision to initiate sporulation.