Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Infection in experimental arthroplasties.

R T Southwood1, J L Rice, P J McDonald

  • 1Flinders Medical Centre, Bedford Park, Adelaide, South Australia.

Clinical Orthopaedics and Related Research
|November 1, 1987
PubMed
Summary

This study shows that Staphylococcus aureus bone infections around implants are best established by direct injection. Antibiotics, especially gentamicin-impregnated bone cement, significantly reduce infection risk in this rabbit model.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The mismatch of narratives and local ecologies in the everyday governance of water access and mosquito control in an urbanizing community.

Health & place·2023
Same author

Recovery from Mosaic Caused by Sorghum Mosaic Virus in Sugarcane and Impact on Yield.

Plant disease·2020
Same author

Risk factors for cerebrospinal fluid shunt infections during an outbreak: a case-control study.

The Journal of hospital infection·2019
Same author

Sugarcane Mosaic Distribution, Incidence, Increase, and Spatial Pattern in Louisiana.

Plant disease·2019
Same author

Active elimination of radio frequency interference for improved signal-to-noise ratio for in-situ NMR experiments in strong magnetic field gradients.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2018
Same author

Clinical outcomes for the obese hospital inpatient: An observational study.

SAGE open medicine·2017

Area of Science:

  • Orthopedic Surgery
  • Infectious Diseases
  • Biomaterials Science

Background:

  • Implant-associated infections are a significant complication in arthroplasty surgery.
  • Developing reliable experimental models is crucial for evaluating preventative strategies.

Purpose of the Study:

  • To investigate the efficacy of different inoculation routes for establishing experimental Staphylococcus aureus infections in rabbit arthroplasties.
  • To assess the effectiveness of antibiotic-loaded bone cement and systemic antibiotics in preventing infection.

Main Methods:

  • Surgical implantation of femoral prostheses and bone cement in rabbits.
  • Inoculation of Staphylococcus aureus via direct medullary injection or intravenous administration.
  • Monitoring infection markers, including erythrocyte sedimentation rate (ESR).

Related Experiment Videos

  • Evaluation of antibiotic interventions (gentamicin-impregnated cement, flucloxacillin, imipenem, rifampicin).

    • Main Results:

    • Direct femoral medullary inoculation of 10^3 Staphylococcus aureus consistently established periprosthetic infection.
    • Intravenous inoculation required significantly higher bacterial doses to induce infection, indicating difficulty in reproducing hematogenous spread.
    • Erythrocyte sedimentation rate (ESR) was identified as the most reliable clinical indicator of infection.
    • Gentamicin-impregnated cement demonstrated substantial protective effects, requiring 60-fold higher bacterial inocula.
    • Flucloxacillin, Imipenem, and Rifampicin also provided significant protection against infection.

    Conclusions:

    • Direct medullary inoculation is a reliable method for creating experimental periprosthetic joint infections.
    • Antibiotic-loaded bone cement and specific systemic antibiotics are highly effective in preventing Staphylococcus aureus arthroplasty infections.
    • This rabbit model provides a valuable platform for testing prophylactic strategies against implant-associated infections.