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Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS
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COVID-19 detection using AIE-active iridium complexes.

Ajay Gupta1, Tarun Adarsh2, Vikas Manchanda3

  • 1School of Physical Sciences, Jawaharlal Nehru University, New Delhi 110067, India. pijus@mail.jnu.ac.in.

Dalton Transactions (Cambridge, England : 2003)
|January 19, 2023
PubMed
Summary
This summary is machine-generated.

A new fluorescence assay detects SARS-CoV-2 RNA in 10 minutes using iridium complexes. This rapid, single-step method offers a promising alternative to complex RT-PCR for point-of-care COVID-19 diagnosis.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • COVID-19 diagnosis relies heavily on RT-PCR, a sensitive but time-consuming method.
  • Current diagnostic limitations hinder rapid point-of-care (POC) testing for SARS-CoV-2.
  • There is a need for faster, simpler diagnostic tools for widespread COVID-19 screening.

Purpose of the Study:

  • To develop a rapid, single-step fluorescence assay for SARS-CoV-2 RNA detection.
  • To utilize aggregation-induced emission (AIE) with iridium complexes for enhanced sensitivity.
  • To create a viable alternative to RT-PCR for POC applications.

Main Methods:

  • Designed iridium complexes as fluorescent probes for SARS-CoV-2 RNA.
  • Employed a single-step fluorescence assay based on AIE principles.
  • Validated the assay's performance using clinical samples at room temperature.

Main Results:

  • The assay provides a "turn-on" fluorescence signal in the presence of SARS-CoV-2 RNA.
  • Results are obtained within 10 minutes at room temperature.
  • Achieved a lower limit of detection (LOD) of 1.84 genome copies/reaction.
  • Demonstrated 90% sensitivity and 80% specificity in clinical samples.

Conclusions:

  • The developed AIE-based fluorescence assay offers a rapid and simple method for SARS-CoV-2 RNA detection.
  • This assay shows potential for effective point-of-care COVID-19 diagnostics.
  • The technology provides a sensitive and specific alternative to traditional RT-PCR methods.