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Inclusion Bodies: Status Quo and Perspectives
1Research Division Integrated Bioprocess Development, TU Wien Institute of Chemical, Environmental, and Bioscience Engineering, Vienna, Austria. julian.kopp@tuwien.ac.at.
Methods in Molecular Biology (Clifton, N.J.)
|January 19, 2023
Summary
Recombinant protein production in E. coli can form inclusion bodies (IBs). Recent advancements make IB processing viable, offering high yields for biopharmaceutical manufacturing.
Area of Science:
- Biotechnology
- Bioprocess Engineering
- Molecular Biology
Background:
- Escherichia coli (E. coli) cultivations for recombinant protein production frequently generate inclusion bodies (IBs).
- Historically, IBs were viewed as undesirable byproducts due to complex and costly purification procedures.
- Current advancements have significantly improved the feasibility and efficiency of IB processing.
Purpose of the Study:
- To review the current state-of-the-art biopharmaceutical processes involving inclusion bodies.
- To identify and discuss the challenges associated with inclusion body processing.
- To highlight recent developments, future perspectives, and alternative solutions in the field.
Main Methods:
- Review of existing literature on E. coli inclusion body production and processing.
- Analysis of industrial biopharmaceutical processes favoring inclusion body formation.
- Comparative assessment of state-of-the-art versus alternative processing strategies.
Main Results:
- E. coli inclusion bodies (IBs) are increasingly favored in industrial bioprocesses due to high space-time yields.
- Overcoming previous obstacles has made IB processing a competitive option against soluble protein production.
- Significant progress has been made in addressing the challenges of IB purification and utilization.
Conclusions:
- Inclusion body production in E. coli is a viable and advantageous strategy for biopharmaceutical manufacturing.
- Continued innovation in processing technologies is crucial for maximizing the benefits of IB formation.
- Alternative processing solutions offer potential for further optimization and cost-effectiveness.
Keywords:
E. coliIB-based productsInclusion bodiesInclusion body processingRecombinant protein production, inclusion body refolding, inclusion body challenges, inclusion body qualityMore Related Videos
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