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Updated: Aug 13, 2025

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Published on: February 5, 2015
Bidirectional Promoter Libraries Enable the Balanced Co-expression of Two Target Genes in E. coli
Julia Jodlbauer1, Lukas Rieder2, Anton Glieder2
1acib - Austrian Centre of Industrial Biotechnology, Graz, Austria.
Abstract:
In this chapter, we present a bidirectional promoter library toolbox to evaluate fast and efficiently the optimal conditions for the balanced co-expression of two target genes. As a proof-of-concept, we demonstrate the co-expression of CYP505x and the GroEL/ES complex, which resulted in noticeably elevated enzyme activity with one of the de-novo-designed promoters of the library. The new toolbox offers a straightforward one-pot cloning approach and is highly modular. As such, the method presented here should be of great interest to any gene co-expression study.
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