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Updated: Aug 13, 2025
09:27
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
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Enzymatic cofactor regeneration systems: A new perspective on efficiency assessment.
Karolina Bachosz1, Jakub Zdarta2, Muhammad Bilal2
1Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland; Department of Biotechnology and Biomedicine, DTU Bioengineering, Technical University of Denmark, Soltofts Plads 227, DK-2800 Kgs. Lyngby, Denmark.
The Science of the Total Environment
|January 19, 2023
Summary
Enzymatic cofactors are essential for biocatalysis but costly. This review summarizes efficient enzymatic cofactor regeneration systems, crucial for industrial applications and sustainable chemical synthesis.
Area of Science:
- Biotechnology
- Enzymology
- Green Chemistry
Background:
- Enzymatic processes offer high specificity, driving industrial demand.
- Enzymatic cofactors are vital for biocatalytic efficiency but are consumed and costly.
- Effective cofactor regeneration systems are critical for economic viability and sustainability.
Purpose of the Study:
- To review current enzymatic cofactor regeneration methods.
- To highlight efficient, specific, and non-toxic regeneration systems.
- To identify future research directions and gaps in cofactor regeneration.
Main Methods:
- Literature review of enzymatic cofactor regeneration strategies.
- Analysis of systems for regenerating nicotinamide dinucleotides, coenzyme A, adenosine 5'-triphosphate, and flavin nucleotides.
- Examination of enzyme immobilization's impact on regeneration system stability and reusability.
Main Results:
- Enzymatic regeneration methods are highly specific, efficient, and non-toxic.
- Successful application in productive synthesis of valuable chemicals, including simultaneous multi-cofactor regeneration.
- Enzyme immobilization enhances stability and reusability of regeneration systems.
Conclusions:
- Enzymatic cofactor regeneration is key to advancing industrial biocatalysis.
- Further research is needed to address literature gaps and optimize regeneration systems.
- Developed systems support sustainable chemical production and enzyme reusability.
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