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Updated: Aug 13, 2025
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
34.2K
Engineering nucleic acid chemistry for precise and controllable CRISPR/Cas9 genome editing
1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecule Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Science Bulletin
|January 20, 2023
Summary
Chemical modifications to guide RNA (gRNA) enhance CRISPR/Cas9 genome editing. These strategies improve the efficacy, specificity, and control of CRISPR/Cas9 technology for therapeutic applications.
Area of Science:
- Biotechnology
- Molecular Biology
- Genetics
Background:
- The CRISPR/Cas9 system offers precise genetic manipulation in mammals.
- Its biomedical applications are expanding, but clinical translation is hindered by off-target effects and low efficiency.
Purpose of the Study:
- To review recent chemical strategies for engineering guide RNA (gRNA) in CRISPR/Cas9.
- To enhance genome editing efficacy, specificity, and controllability.
Main Methods:
- Summarizing chemical modifications applied to gRNA.
- Analyzing strategies to improve CRISPR/Cas9 system performance.
Main Results:
- Chemical engineering of gRNA can significantly boost CRISPR/Cas9 efficacy and specificity.
- New approaches address key challenges in genome editing accuracy and on-target efficiency.
Conclusions:
- Chemical strategies for gRNA modification show great potential for advancing CRISPR/Cas9 technology.
- These innovations aim to bridge the gap between laboratory research and clinical applications.
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