SDHB reduction promotes oral lichen planus by impairing mitochondrial respiratory function
Hui Zhang1, Beiyun Xu1, Jin Liu1
1Department of Stomatology, Huashan Hospital, Fudan University, Shanghai, China.
Annals of Translational Medicine
|January 20, 2023
Summary
Reduced succinate dehydrogenase B (SDHB) expression in oral lichen planus (OLP) impairs mitochondrial function, potentially increasing oral cancer risk. Restoring SDHB levels may reverse these detrimental effects.
Area of Science:
- Biochemistry
- Cell Biology
- Oncology
Background:
- Oral lichen planus (OLP) is a chronic inflammatory condition with malignant transformation potential.
- Succinate dehydrogenase B (SDHB) is linked to oral squamous cell carcinoma (OSCC) carcinogenesis, but its role in OLP is unclear.
Purpose of the Study:
- To investigate the expression and function of SDHB in OLP.
- To elucidate the mechanism by which SDHB influences OLP pathogenesis and potential malignant transformation.
Main Methods:
- Immunohistochemistry (IHC), qRT-PCR, and Western Blot (WB) were used to assess SDHB expression in OLP tissues and cell models.
- Functional assays including ATP, ROS, mitochondrial membrane potential (MMP), and glucose uptake were performed to evaluate SDHB's role in mitochondrial injury and bioenergetics.
Main Results:
- SDHB mRNA and protein levels were significantly decreased in OLP patients, correlating with succinate accumulation.
- Decreased SDHB in OLP cell models enhanced HIF-1α activity, induced mitochondrial dysfunction, altered bioenergetics, and promoted cytokine release.
- SDHB overexpression reversed these pathological changes and modulated bioenergetic metabolism.
Conclusions:
- Reduced SDHB expression is a key factor in OLP development by impairing mitochondrial respiratory function.
- Targeting SDHB may offer a therapeutic strategy to mitigate OLP progression and reduce oral cancer risk.
Related Concept Videos
01:46Electron Transport Chain: Complex I and II
14.8K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
14.8K
02:23Alcohols from Carbonyl Compounds: Reduction
10.6K
Reduction is a simple strategy to convert a carbonyl group to a hydroxyl group. The three major pathways to reduce carbonyls to alcohols are catalytic hydrogenation, hydride reduction, and borane reduction.
Catalytic hydrogenation is similar to the reduction of an alkene or alkyne by adding H2 across the pi bond in the presence of transition metal catalysts like Raney Ni, Pd–C, Pt, or Ru. Aldehydes and ketones can be reduced by this method, often under mild to moderate heat (25–100°C) and...
Catalytic hydrogenation is similar to the reduction of an alkene or alkyne by adding H2 across the pi bond in the presence of transition metal catalysts like Raney Ni, Pd–C, Pt, or Ru. Aldehydes and ketones can be reduced by this method, often under mild to moderate heat (25–100°C) and...
10.6K
01:19Acid Halides to Alcohols: LiAlH4 Reduction
3.0K
Acid halides are reduced to alcohols in the presence of a strong reducing agent like lithium aluminum hydride.
The mechanism proceeds in three steps. First, the nucleophilic hydride ion attacks the carbonyl carbon of the acid halide to form a tetrahedral intermediate. Next, the carbonyl group is re-formed, and the halide ion departs as a leaving group, generating an aldehyde. A second nucleophilic attack by the hydride yields an alkoxide ion, which, upon protonation, gives a primary alcohol as...
The mechanism proceeds in three steps. First, the nucleophilic hydride ion attacks the carbonyl carbon of the acid halide to form a tetrahedral intermediate. Next, the carbonyl group is re-formed, and the halide ion departs as a leaving group, generating an aldehyde. A second nucleophilic attack by the hydride yields an alkoxide ion, which, upon protonation, gives a primary alcohol as...
3.0K
01:20Sulfur Assimilation
52
Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...
52