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Simultaneous colorimetric detection of nephrolithiasis biomarkers using a microfluidic paper-based analytical device
Manassawee Janrod1, Monpichar Srisa-Art1
1Electrochemistry and Optical Spectroscopy Center of Excellence, Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand. monpichar.s@chula.ac.th.
Analytical Methods : Advancing Methods and Applications
|January 20, 2023
Summary
A new microfluidic paper-based analytical device (μPAD) enables simultaneous detection of urinary oxalate, citrate, and uric acid (UA) for kidney stone diagnosis. This fast, portable tool offers reliable on-site analysis with minimal sample and reagent use.
Area of Science:
- Analytical Chemistry
- Biomarker Detection
- Point-of-Care Diagnostics
Background:
- Nephrolithiasis (kidney stones) diagnosis relies on monitoring urinary biomarkers like oxalate, citrate, and uric acid (UA).
- Existing diagnostic methods can be time-consuming, require specialized equipment, and are not suitable for rapid, on-site analysis.
- There is a need for a simple, efficient, and portable tool for simultaneous determination of these key urinary biomarkers.
Purpose of the Study:
- To develop a microfluidic paper-based analytical device (μPAD) for the simultaneous colorimetric determination of urinary oxalate, citrate, and uric acid (UA).
- To validate the accuracy, precision, and efficiency of the developed μPAD for on-site urine analysis.
- To provide a cost-effective and rapid diagnostic tool for nephrolithiasis-related biomarker monitoring.
Main Methods:
- Fabrication of a microfluidic paper-based analytical device (μPAD).
- Colorimetric detection assays utilizing enzymatic reactions (oxalate oxidase, uricase) and indicator displacement assay (copper murexide complex for citrate).
- Simultaneous determination of oxalate, citrate, and UA in urine samples within 25 minutes.
Main Results:
- The μPAD achieved simultaneous determination of oxalate, citrate, and UA in urine within 25 minutes.
- Linear ranges were established as 2-40 mg L⁻¹ (oxalate), 5-150 mg L⁻¹ (UA), and 5-45 mg L⁻¹ (citrate).
- Detection limits were 0.6 mg L⁻¹ (oxalate), 2.9 mg L⁻¹ (UA), and 3.1 mg L⁻¹ (citrate), with %RSD < 6.4% and recovery rates of 81.0-109.8%.
Conclusions:
- The developed μPAD is a simple, fast, inexpensive, and portable tool for simultaneous determination of urinary oxalate, citrate, and UA.
- The device demonstrates acceptable accuracy and precision, making it suitable for reliable on-site analysis.
- This μPAD holds significant promise for improved diagnostics and management of nephrolithiasis.
