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A colorimetric paper-based immunosensor for high-sensitivity C-reactive protein (hs-CRP) detection.

Duangtip Lawanstien1, Pakakan Ngernpradab1, Monpichar Srisa-Art1

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A novel paper-based immunosensor detects high-sensitivity C-reactive protein (hs-CRP), a key cardiovascular disease risk marker. This simple, portable device offers reliable hs-CRP quantification for effective CVD risk assessment, especially in resource-limited settings.

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Immunosensor Technology

Background:

  • Cardiovascular diseases (CVDs) pose a significant global health burden.
  • High-sensitivity C-reactive protein (hs-CRP) is a validated biomarker for predicting CVD risk.
  • Accurate and accessible hs-CRP detection methods are crucial for early risk assessment.

Purpose of the Study:

  • To develop a simple, portable, and instrument-free colorimetric immunosensor for hs-CRP detection.
  • To utilize a paper-based analytical device (PAD) integrated with immunomagnetic separation (IMS).
  • To establish a clinically relevant detection range and high precision for hs-CRP quantification.

Main Methods:

  • Development of a sandwich immunoassay utilizing magnetic beads for immunomagnetic separation (IMS).
  • Immobilization of capture antibodies on magnetic beads and biotinylation of detection antibodies.
  • Enzymatic colorimetric detection using β-galactosidase (βgal) and a sensitive substrate (CRPG).

Main Results:

  • Achieved a limit of detection (LOD) of 0.10 mg L-1 for hs-CRP.
  • Established a clinically relevant detection range of 0.30-3.0 mg L-1.
  • Demonstrated high precision (inter- and intraday % RSD < 5%) and reliability in human serum analysis.

Conclusions:

  • The developed PAD-based immunosensor provides a simple, portable, and instrument-free method for hs-CRP detection.
  • The sensor exhibits sufficient sensitivity and precision for CVD risk assessment.
  • This technology holds promise for developing cost-effective screening kits in resource-limited settings.