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QuPath Analysis for CD30+ Cutaneous T-Cell Lymphoma.

Cassandra Cieslak1,2, Christina Mitteldorf3, Tanja Krömer-Olbrisch1,2

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The American Journal of Dermatopathology
|January 20, 2023
PubMed
Summary
This summary is machine-generated.

Quantitative image analysis using QuPath software offers an objective approach to assessing CD30+ expression in mycosis fungoides. This digital pathology method reduces subjectivity compared to manual assessment by histopathologists, improving diagnostic accuracy for targeted therapies.

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Area of Science:

  • Digital Pathology
  • Oncology
  • Immunohistochemistry

Background:

  • Mycosis fungoides is the most common cutaneous T-cell lymphoma.
  • CD30 expression is a target for brentuximab vedotin therapy.
  • Current diagnostic methods rely on subjective manual assessment of immunohistochemical (IHC) staining.

Purpose of the Study:

  • To evaluate the objectivity of QuPath software for quantitative analysis of CD30 expression in mycosis fungoides.
  • To compare QuPath's quantitative results with manual assessments by dermatopathologists.

Main Methods:

  • Ten mycosis fungoides patient samples with varying CD30 expression were analyzed.
  • IHC staining for CD3 and CD30 was performed, followed by slide scanning.
  • Quantitative analysis was conducted using QuPath (version 2.1) and compared to manual assessments by three dermatopathologists.

Main Results:

  • Manual assessments of CD30+/CD3+ cells showed high interobserver variability (mean CV, 0.46).
  • QuPath analysis demonstrated excellent separation of stained cells from other tissue components.
  • QuPath results strongly correlated with the mean estimates of the histopathologists (Pearson-R 0.93).

Conclusions:

  • Quantitative image analysis, like QuPath, significantly reduces interobserver variability in IHC marker evaluation.
  • Objective assessment is crucial for clinical routine and therapeutic studies involving targeted molecules.
  • Digital pathology offers a more reliable method for quantifying biomarkers in cutaneous lymphomas.