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Defining Two Chemosensory Arrays in Shewanella oneidensis
Emma M Fortier1, Sophie Bouillet1,2, Pascale Infossi1
1Laboratoire de Bioénergétique et Ingénierie des Protéines, Institut de Microbiologie de la Méditerranée, Centre National de la Recherche Scientifique, Aix-Marseille Université, 13402 Marseille, France.
Biomolecules
|January 21, 2023
Summary
Two Shewanella oneidensis chemoreceptors, Aer2so and McpAso, link the Che1 and Che3 signaling systems. This interconnection allows for a global bacterial survival response.
Area of Science:
- Microbiology
- Bacterial Chemosensory Systems
- Molecular Biology
Background:
- Shewanella oneidensis possesses two chemosensory systems, Che1 and Che3, and 27 chemoreceptors.
- Che3 is primarily involved in chemotaxis, while Che1 may play a role in RpoS post-translational regulation.
Purpose of the Study:
- To investigate the roles of specific chemoreceptors in Shewanella oneidensis signaling pathways.
- To determine how chemoreceptors Aer2so and McpAso interact with the Che1 and Che3 systems.
Main Methods:
- Genetic analysis of chemoreceptor function.
- Cellular localization studies of chemoreceptor complexes.
- Investigation of signaling pathways involving CheA1 and CheW1.
Main Results:
- Chemoreceptors Aer2so and McpAso form distinct signaling units.
- Aer2so and McpAso signal to the Che1 and Che3 systems, respectively.
- Aer2so forms a dynamic cytoplasmic complex that localizes to cellular poles and the center.
Conclusions:
- The Che1 and Che3 systems are interconnected through chemoreceptors Aer2so and McpAso.
- These chemoreceptors facilitate a coordinated cellular response crucial for bacterial survival.
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