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Updated: Aug 13, 2025

High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity
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Ligand Binding Properties of Odorant-Binding Protein OBP5 from Mus musculus

Lucie Moitrier1, Christine Belloir1, Maxence Lalis2

  • 1Centre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université Bourgogne Franche-Comté, F-21000 Dijon, France.

Biology
|January 21, 2023
PubMed

Insights

This study characterizes mouse odorant-binding protein 5 (mOBP5), revealing its specific binding properties for certain odorants. These findings contribute to understanding olfactory mechanisms and odorant transport in mammals.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Olfactory Science

Background:

  • Odorant-binding proteins (OBPs) are crucial for olfaction, facilitating odorant transport to receptors.
  • Their precise roles and binding specificities require detailed functional characterization.

Purpose of the Study:

  • To functionally characterize mouse odorant-binding protein 5 (mOBP5).
  • To elucidate the binding properties and molecular determinants of mOBP5.

Main Methods:

  • Recombinant expression and purification of mOBP5.
  • Circular dichroism spectroscopy, gel filtration, and fluorescence-based binding assays.
  • Isothermal titration calorimetry (ITC), homology modeling, and molecular docking.

Main Results:

  • mOBP5 was successfully expressed and purified.
  • Fluorescence and ITC revealed micromolar affinity binding to specific odorants.
  • Homology modeling identified key residues responsible for odorant binding.

Conclusions:

  • mOBP5 exhibits specific odorant-binding capabilities.
  • Structural insights into mOBP5 binding provide a basis for understanding olfactory receptor interactions.
Keywords:
affinitycircular dichroismfluorescent assayisothermal microcalorimetrylipocalinodorantodorant-binding proteinpurification

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