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The Arteriovenous AV Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering

The Arteriovenous AV Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering

Published on: November 2, 2016

A Novel Window into Angiogenesis-Intravital Microscopy in the AV-Loop-Model.

Ravikumar Vaghela¹, Andreas Arkudas¹, Daniel Gage²

¹Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany.

|January 21, 2023

Summary

Researchers developed novel in vivo imaging chambers to visualize cellular processes in the arteriovenous (AV) loop model. This advancement enhances understanding of vascular development for tissue engineering applications.

Keywords:

GelMA arteriovenous loop intravital microscopy tissue engineering

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Published on: September 30, 2021

Area of Science:

Biomedical Engineering
Regenerative Medicine
Vascular Biology

Background:

Current in vivo models have limitations in studying vascular development.
Understanding vascularization is crucial for engineered tissue constructs.
The arteriovenous (AV) loop model offers a platform for studying vascularization.

Purpose of the Study:

To develop novel in vivo imaging chambers for live visualization of cellular processes.
To enable detailed study of vascular development in the AV loop model.
To overcome limitations of existing experimental designs.

Main Methods:

Two types of intravital microscopy (IVM) chambers were designed and fabricated.
Chambers were implanted in rats using different fixation methods (skin and subcutaneous).
Modified gelatin hydrogel was used as a matrix, and fluorescent dyes were employed for visualization.

Main Results:

Chamber A supported AV loop function for two weeks, enabling leukocyte trafficking visualization.
Chamber B allowed examination of microvascular development in the AV loop for 21 days.
Microvascular outgrowth was quantified using Fiji-ImageJ software.

Conclusions:

The developed IVM chambers provide a unique tool for studying vascular development in the AV loop model.
Combining both chamber types offers a comprehensive approach to understanding vascularization.
This research advances the field of tissue engineering by improving in vivo study capabilities.