Solid-phase extraction of chloramphenicol with graphitized carbon black.

Area of Science:

• Analytical Chemistry
• Chromatography
• Biomedical Analysis

Background:

• Chloramphenicol (CP) is a widely used antibiotic.
• Accurate quantification of CP in biological fluids is crucial for therapeutic drug monitoring and toxicological studies.
• Existing methods may require optimization for efficiency and sensitivity.

Purpose of the Study:

• To evaluate the sorption and desorption properties of graphitized carbon black (GCB) for chloramphenicol (CP) enrichment.
• To develop and optimize a solid-phase extraction (SPE) method for CP analysis in biological samples.
• To determine the optimal parameters for GCB-based SPE of CP.

Main Methods:

• Sorption and desorption studies of CP on GCB.
• Evaluation of different organic solvents for CP elution, focusing on methanol.
• Determination of optimal GCB packing amount, eluent volume, and flow rate using breakthrough and elution curves.
• Optimization of column inner diameter based on CP recovery.
• Application of the developed SPE method to serum samples.

Main Results:

• CP distribution strongly favored GCB over water, indicating efficient sorption.
• Methanol exhibited the lowest adsorption coefficient among tested organic solvents, making it a suitable eluent.
• Optimal GCB packing, eluent volume, and flow rate were determined.
• Column inner diameter was optimized to maximize CP recovery.
• The GCB-SPE method was successfully applied for CP assay in serum.

Conclusions:

• Graphitized carbon black is an effective sorbent for chloramphenicol enrichment from biological matrices.
• The developed solid-phase extraction method provides a robust approach for chloramphenicol analysis in serum.
• Optimized GCB-SPE parameters enhance the efficiency and reliability of chloramphenicol quantification.