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Updated: Sep 25, 2026

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Solid-phase extraction of chloramphenicol with graphitized carbon black
Abstract:
The sorption and desorption properties of graphitized carbon black (GCB) were evaluated for enrichment of highly polarizable chloramphenicol (CP) from biological fluids. The distribution of CP between GCB and water lay strongly toward GCB. Among the organic solvents examined, methanol gave the lowest adsorption coefficient. The optimum packing amount of GCB, and the optimum volume and flow-rate of the eluent were deduced from the breakthrough curve and methanol elution curve, respectively. The inner diameter of GCB extraction columns was chosen from the sorption efficiency curve obtained by plotting CP recovery against column inner diameter. The solid-phase extraction method using GCB was applied to the assay of CP in serum samples in the concentration range of 5 to 50 micrograms/ml.
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