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Purification of saikosaponins a, c and d. Application of large-scale reversed high-performance liquid chromatography
1Chromatographic Division, Kurita Water Industries Ltd., Tokyo, Japan.
Journal of Chromatography
|July 29, 1987
Abstract:
The preparative separation of saikosaponins a, c, and d from Bupleurum falcatum was performed by high-performance liquid chromatography on a 100 X 11 cm I.D. axially compressed column, packed with 20-micron ODS silica gel using a step gradient with mixtures of acetonitrile and water as the mobile phase. Gram quantities of pure compounds were obtained in a single run.