Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Comparing Experimental Results: Student's t-Test01:09

Comparing Experimental Results: Student's t-Test

1.6K
The t-test is a statistical method used to compare the sample mean with a population mean or compare two means from two data sets. The test statistic is calculated from the standard deviation, mean, and number of measurements in the data set at a selected confidence interval and then compared to a table of critical values at this confidence level. If the test statistic is smaller than the critical value, the null hypothesis is accepted. In this case, we state that the difference between the...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A call for national standardization and cut-off validation and not replacement of HbA1c.

The Lancet regional health. Southeast Asia·2026
Same author

A systematic review of total IgE reference intervals - A 2024 update.

Clinica chimica acta; international journal of clinical chemistry·2024
Same author

Use of Partial Least Squares improves the efficacy of removing unwanted variability in differential expression analyses based on RNA-Seq data.

Genomics·2018
Same author

A Young Male with Bilateral Gynecomastia.

Clinical chemistry·2018
Same author

Detection and accurate false discovery rate control of differentially methylated regions from whole genome bisulfite sequencing.

Biostatistics (Oxford, England)·2018
Same author

Severe Hyponatremia with Hypouricemia in a Patient with Medullary Hemorrhage: A Case Report.

EJIFCC·2016

Related Experiment Video

Updated: Aug 13, 2025

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method
14:48

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

Published on: July 6, 2012

43.7K

Analytical Variation Between Two Different TSH Reagents from the Same Manufacturer.

Sudhesna Mohapatra1, Sutirtha Chakraborty2

  • 1Department of Biochemistry, Humain Health, Bengaluru, Karnataka 560102 India.

Indian Journal of Clinical Biochemistry : IJCB
|January 23, 2023
PubMed
Summary
This summary is machine-generated.

Thyroid stimulating hormone (TSH) immunoassay results vary significantly between kits, impacting diagnosis. A Siemens Healthineers study revealed a bias, potentially misclassifying hypothyroidism cases.

Keywords:
HarmonizationHypothyrodismImmunoassayMethod comparisonTSHThyroid stimulating hormone

More Related Videos

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
04:39

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Published on: March 17, 2023

1.8K
Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research
05:25

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Published on: July 14, 2023

1.5K

Related Experiment Videos

Last Updated: Aug 13, 2025

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method
14:48

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

Published on: July 6, 2012

43.7K
Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
04:39

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Published on: March 17, 2023

1.8K
Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research
05:25

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

Published on: July 14, 2023

1.5K

Area of Science:

  • Clinical Chemistry
  • Immunoassay Technology
  • Endocrinology

Background:

  • Thyroid stimulating hormone (TSH) immunoassays are crucial for diagnosing thyroid dysfunction.
  • Variability in TSH assay results across different platforms and kit generations can affect clinical decisions.
  • Consistent and accurate TSH measurement is essential for proper patient management.

Purpose of the Study:

  • To compare the performance of two TSH immunoassays from the same manufacturer, Siemens Healthineers.
  • To identify and quantify any discrepancies or bias between the TSH assay methods.
  • To assess the potential clinical impact of observed assay differences on thyroid dysfunction diagnosis.

Main Methods:

  • A method comparison study was conducted using two TSH immunoassay kits from Siemens Healthineers.
  • Patient samples were analyzed using both TSH assay methods.
  • Statistical analysis was performed to determine the bias and proportional error between the two assays.

Main Results:

  • A significant proportional error was detected between the two TSH assays.
  • A bias of -3.71 mIU/L was observed, indicating higher TSH values with one assay compared to the TSH3-Ultra kit.
  • The identified bias suggests that TSH assay results may lead to overestimation of TSH levels.

Conclusions:

  • The observed proportional error and bias between TSH assays can lead to misinterpretation of results.
  • This discrepancy may result in incorrect categorization of patients with hypothyroidism and subclinical hypothyroidism.
  • In-house validation and method comparison studies are crucial to ensure assay performance and prevent clinical errors in thyroid dysfunction evaluation.