Related Experiment Video
Updated: Aug 13, 2025
12:44
The In ovo CAM-assay as a Xenograft Model for Sarcoma
Published on: July 17, 2013
25.6K
Chondrosarcoma with Target-Like Chondrocytes: Update on Molecular Profiling and Specific Morphological Features
1Institute of Pathology, First Faculty of Medicine, Charles University and General University Hospital in Prague, and Institute of Postgraduate Studies, Prague, Czech Republic.
Folia Biologica
|January 23, 2023
Summary
This study reveals unique chondrosarcoma features, identifying target-like chondrocytes with specific perichondrocytic rings. A novel FN1-FGFR2 fusion transcript was discovered in these rare bone tumors.
Area of Science:
- Oncology
- Histopathology
- Molecular Biology
Background:
- Chondrosarcomas are malignant tumors of cartilage.
- Target-like chondrocytes represent a rare histological subtype.
- Understanding their unique features is crucial for diagnosis and treatment.
Purpose of the Study:
- To perform the first histological and molecular analysis of chondrosarcomas with target-like chondrocytes.
- To compare these tumors with conventional chondrosarcomas and enchondromas.
- To identify the molecular underpinnings of this rare chondrosarcoma variant.
Main Methods:
- Histological and immunohistochemical analysis of tumor samples.
- Safranin O/Fast green staining for matrix components.
- Ultrastructural examination of pericellular structures.
- Next-generation sequencing (NGS) for molecular profiling.
Main Results:
- Target-like chondrocytes exhibit eosinophilic, APAS-positive perichondrocytic rings ('baskets').
- Immunohistochemistry showed positivity for vimentin, factor XIIIa, cyclin D1, osteonectin, Bcl-2, p53, and p16.
- Pericellular rings contained type VI collagen, distinct from intercellular type II collagen.
- NGS identified a novel FN1-FGFR2 fusion transcript at the RNA level.
Conclusions:
- Chondrosarcomas with target-like chondrocytes possess distinct histological and ultrastructural features.
- The identified FN1-FGFR2 fusion transcript may play a role in the pathogenesis of these rare tumors.
- Further research is warranted to elucidate the clinical significance of this finding.
