Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microenvironments01:22

Microenvironments

Microorganisms inhabit highly localized spaces known as microenvironments, which are defined by distinct physical and chemical characteristics. These include oxygen concentration, pH, temperature, light availability, and nutrient levels. The conditions within a microenvironment can differ markedly from those in the surrounding area and significantly influence microbial growth, metabolism, and community structure.Microenvironments often display sharp physicochemical gradients over small spatial...
Microbial Mats01:25

Microbial Mats

Microbial communities forming biofilms and mats represent complex, spatially structured ecosystems where metabolic processes are stratified according to light, oxygen, and nutrient gradients. Biofilms are initial colonization stages, only a few millimeters thick, while mature microbial mats can reach centimeter-scale thickness and display intricate vertical organization. Their structural and functional heterogeneity allows microorganisms to occupy distinct ecological niches within a few...
Marine Microbial Ecology01:30

Marine Microbial Ecology

Marine microbial ecosystems are shaped by distinct physicochemical limits, including high salinity, low nutrient availability, and fluctuating oxygen levels. These conditions favor smaller microbial cell sizes, which maximize their surface-to-volume ratio for efficient nutrient uptake.Microbial activity and community composition are closely linked to biogeochemical cycles, particularly in dynamic environments like estuaries, where halotolerant microbes thrive in response to variable salinity...
Microbial Wastewater Treatment01:30

Microbial Wastewater Treatment

Microbial communities in aquatic ecosystems play a key role in the natural breakdown of contaminants introduced through domestic and industrial effluents. Acting as biological catalysts, these microbes change and mineralize a wide range of organic and inorganic pollutants under different redox conditions.In oxygen-rich surface waters, aerobic heterotrophs lead organic matter breakdown, using oxygen as the terminal electron acceptor to efficiently oxidize substrates to carbon dioxide and water.
Microbial Corrosion01:24

Microbial Corrosion

Microbiologically Influenced Corrosion (MIC) is a significant form of material degradation caused by the metabolic activities of microorganisms. This phenomenon poses substantial challenges across various industries, including oil and gas, maritime, and water treatment sectors.MIC occurs when microorganisms, such as bacteria, archaea, and fungi, colonize metal surfaces, forming biofilms that alter the local electrochemical environment. These biofilms can lead to the production of corrosive...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Considering internal conflict in the face of natural product biosynthesis and biosynthetic gene cluster evolution.

Essays in biochemistry·2026
Same author

Genome mining of amylases and amylase inhibitors from <i>Streptomyces</i>.

Microbial genomics·2026
Same author

Assessing mature and immature collagen in tonsillar tissue using second harmonic generation microscopy.

Journal of microscopy·2026
Same author

Colony politics to chemical warfare: phenotypic plasticity in Streptomyces.

Essays in biochemistry·2026
Same author

Mesolens imaging in microbiology.

Essays in biochemistry·2026
Same author

Building capacity in imaging data management.

Journal of microscopy·2026

Related Experiment Video

Updated: Jun 25, 2026

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
09:45

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

Published on: January 25, 2017

19.7K

Addressing multiscale microbial challenges using the Mesolens.

Liam M Rooney1, Beatrice Bottura1, Katherine Baxter1

  • 1Strathclyde Institute for Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.

Journal of Microscopy
|January 24, 2023
PubMed
Summary
This summary is machine-generated.

The Mesolens offers unparalleled high-resolution imaging for large microbial samples, revolutionizing microbiology research. This advanced microscopy technique provides a large field-of-view ideal for studying biofilms and infected tissues.

Keywords:
biofilmhigh‐content imagingmesoscopic imagingmicrobiology

More Related Videos

Generating Controlled, Dynamic Chemical Landscapes to Study Microbial Behavior
10:07

Generating Controlled, Dynamic Chemical Landscapes to Study Microbial Behavior

Published on: January 31, 2020

6.2K
Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
12:32

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

Published on: November 25, 2020

6.6K

Related Experiment Videos

Last Updated: Jun 25, 2026

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
09:45

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

Published on: January 25, 2017

19.7K
Generating Controlled, Dynamic Chemical Landscapes to Study Microbial Behavior
10:07

Generating Controlled, Dynamic Chemical Landscapes to Study Microbial Behavior

Published on: January 31, 2020

6.2K
Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
12:32

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

Published on: November 25, 2020

6.6K

Area of Science:

  • Microbiology
  • Microscopy
  • Bioimaging

Background:

  • Microbial specimens like biofilms and infected tissues are often collected as large, multimillimetre-sized samples.
  • Observing microscopic details within these mesoscale samples is crucial for answering key scientific questions.
  • Existing optical microscopes struggle to provide both a large field-of-view and high-resolution imaging for such samples.

Purpose of the Study:

  • To review the development and applications of the Mesolens in microbiology.
  • To highlight the Mesolens' unique capabilities for imaging mesoscale microbial specimens.
  • To discuss the potential impact of Mesolens technology on future microbiological research.

Main Methods:

  • Review of existing literature on Mesolens development and application.
  • Demonstration of Mesolens imaging capabilities on various microbial specimens (e.g., biofilms, tissue samples).
  • Analysis of Mesolens' optical properties (low magnification, high numerical aperture).

Main Results:

  • The Mesolens provides a unique combination of low magnification and high numerical aperture.
  • This enables high-resolution imaging across a large field-of-view, ideal for mesoscale microbial samples.
  • Current applications showcase the Mesolens' effectiveness in microbial imaging.

Conclusions:

  • The Mesolens is an ideal imaging method for mesoscale microbial specimens, surpassing other optical microscopes.
  • Its unique optical properties facilitate detailed observation of microscopic features in large samples.
  • The Mesolens holds significant potential to advance various fields within microbiology.