Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Controlled Precipitation and Microcoating Formation of Shellac via Sequential Ethanol-Water Solvent Exchange.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Skeletal Stabilization After Sagittal Split Osteotomy Using a Biodegradable Osteosynthesis System: A Pilot Study.

The Journal of craniofacial surgery·2026
Same author

Correction: Appearance-related attentional bias is associated with dysmorphic appearance concern in individuals with jaw deformity: an eye-tracking study.

Frontiers in psychiatry·2026
Same author

Current status of multilayer neutron interferometry with gaseous samples at J-PARC.

Journal of applied crystallography·2026
Same author

Appearance-related attentional bias is associated with dysmorphic appearance concern in individuals with jaw deformity: an eye-tracking study.

Frontiers in psychiatry·2026
Same author

Efficient recovery of chlorogenic acid and rutin from Ficus erecta leaves using liquid carbon dioxide-ethanol-water extraction with and without ultrasonic irradiation.

Ultrasonics sonochemistry·2026

Related Experiment Video

Updated: Aug 12, 2025

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
07:15

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

11.6K

Transcriptome profiles associated with human periodontal ligament differentiation.

Yuji Takahashi1, Rika Yasuhara2, Junichi Tanaka2

  • 1Department of Orthodontics, School of Dentistry, Showa University, Tokyo, 142-8555, Japan; Division of Pathology, Department of Oral Diagnostic Sciences, School of Dentistry, Showa University, Tokyo, 142-8555, Japan.

Journal of Oral Biosciences
|January 24, 2023
PubMed
Summary

Researchers identified 37 transcription factors that may induce periodontal ligament (PDL) cell differentiation from human pluripotent stem cells (hPSCs). Targeting these factors and the SMAD signaling pathway shows promise for regenerative medicine.

Keywords:
Neural crest cellsPeriodontal ligamentPluripotent stem cellsPrimary cultureRNA sequence

More Related Videos

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model
06:14

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

Published on: February 17, 2023

4.2K
Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

3.8K

Related Experiment Videos

Last Updated: Aug 12, 2025

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
07:15

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

11.6K
Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model
06:14

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

Published on: February 17, 2023

4.2K
Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

3.8K

Area of Science:

  • Stem Cell Biology
  • Periodontal Regeneration
  • Molecular Biology

Background:

  • Tissue differentiation is a complex process governed by transcription factors.
  • Periodontal ligament (PDL) cells are crucial for tooth support and regeneration.
  • Human pluripotent stem cells (hPSCs) offer a potential source for regenerative therapies.

Purpose of the Study:

  • To identify candidate transcription factors capable of inducing PDL cell differentiation.
  • To explore the potential of hPSCs as a source for generating PDL cells.
  • To investigate gene expression profiles for PDL cell differentiation induction.

Main Methods:

  • Human PDL tissues were cultured using the explant method.
  • RNA sequencing (RNA-seq) was employed to profile gene expression in PDL cells.
  • PDL cell gene expression was compared to undifferentiated hiPSCs and hESC-derived neural crest (NC) cells.

Main Results:

  • Primary cultured PDL cells displayed characteristic morphology and expressed PDL-specific markers.
  • Gene expression profiles of PDL cells closely resembled hESC-derived NC cells.
  • Thirty-seven transcription factors were found to be upregulated in PDL cells, with enrichment in the SMAD 2/3 pathway.

Conclusions:

  • Thirty-seven upregulated transcription factors were identified in PDL cells compared to hESC-derived NC cells.
  • Modulating these identified transcription factors and the SMAD signaling pathway may induce PDL cell differentiation from hPSC-derived NC cells.
  • This research provides a foundation for developing novel strategies for periodontal regeneration using stem cells.