Polyethyleneimine-induced fluorescence enhancement strategy for AIEgen: the mechanism and application

Aggregation-induced emission luminogens (AIEgens) are attracting extensive research attention in the biosensor fields. Herein, we report a new polyethyleneimine (PEI)-induced strategy for enhancing luminescence of TCBPE (an AIEgen) to promote its development in biosensor. The copolymer dots (TCBPE-PEI) with high quantum yield (39.7%) and outstanding stability were synthesized via a one-pot method. The fluorescence enhancement mechanism based on the PEI strategy originated from the restriction of intramolecular motions of TCBPEs and the form of donor-acceptor structures to decrease the inherent energy bandgap. Benefiting from chelating property of TCBPE-PEI by Cu²⁺, a fluorescence-quenching sensor for Cu²⁺ detection was developed based on the fluorescence quenching of the electron transfer effect. Especially, a good linear range of 10-250 nM with a low limit of detection 1.1 nM was achieved, and it was further applied in samples successfully. The current work provides a novel approach to fabricate AIEgen biosensors and shows great potential in Cu²⁺ detection.