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A visual bi-layer indicator based on mulberry anthocyanins with high stability for monitoring Chinese mitten crab
Xiaowei Huang1, Liuzi Du2, Zhihua Li2
1School of Food and Biological Engineering, School of Agricultural Equipment Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China; College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, 128 North Railway Street, Gulou District, Nanjing 210023, China.
Abstract:
In this study, a bilayer film (BIF) was fabricated to improve the stability of an anthocyanin-based freshness indicator film. The sensor layer consists of gellan gum (GG) and mulberry anthocyanin (MAE) for freshness indication. The oxygen barrier layer was constructed from chitosan (CS), polyvinyl alcohol (PVA), sodium alginate (SA), and pullulan (Pu) to the protection of MAE from oxidation. The highest antioxidant activity of BIF was 91.28 %. BIF was used to monitor the Chinese mitten crab freshness. The total volatile basic nitrogen (TVB-N) level was increased to 31.23 mg/100 g on day 8, and the color of the indicator presented a visible change from pink to dark green. The acquired results revealed a good correlation between TVB-N, pH, and color change of the indicator. The research indicated that the BIF was applied for freshness monitoring of Chinese mitten crab and displayed significant color changes that would be effective in commercial environments.
