Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

92.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.0K
Sanger Sequencing01:57

Sanger Sequencing

755.6K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
755.6K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

72
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
72
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

7.8K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.8K
RNA-seq03:21

RNA-seq

10.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.2K
Combination Therapies and Personalized Medicine02:50

Combination Therapies and Personalized Medicine

5.0K
Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

UGT1A1 genotype testing for irinotecan: A guideline developed by the UK Centre of Excellence in Regulatory Science and Innovation in Pharmacogenomics (CERSI-PGx).

British journal of clinical pharmacology·2026
Same author

Vanishing liver metastases in pancreatic cancer: A case report.

Oncology letters·2026
Same author

Competence is not enough: organisational readiness for safe ECMO nursing care.

Intensive & critical care nursing·2026
Same author

Dendrimer-nanoparticle (DEP) delivery of cabazitaxel (DEP-cabazitaxel): A first-in-human phase 1/2 trial in patients with advanced solid tumours.

European journal of cancer (Oxford, England : 1990)·2026
Same author

Associations Between Routinely Measured Biomarkers and Pancreatic Cancer Risk in Individuals with Type 2 Diabetes Pre- and Post-Diagnosis.

Cancers·2026
Same author

Fianlimab, a human lymphocyte activation gene-3 monoclonal antibody, in combination with cemiplimab: Tumor-specific expansion cohorts in advanced malignancies.

Cancer·2026
Same journal

Letter to the Editor: 'Life expectancy of UK physicians in the early 21st century: An analysis of 1,000 fellows from the Royal College of Physicians' Munk's Roll'.

Clinical medicine (London, England)·2026
Same journal

Finerenone Exposure and Ischemic Stroke in Patients with Type 2 Diabetes and Chronic Kidney Disease: A Propensity Score-Matched Cohort Study.

Clinical medicine (London, England)·2026
Same journal

Prevalence and predictive factors of chronic kidney disease among individuals with cardiometabolic risk factors: A multicenter cross sectional study, North East Ethiopia.

Clinical medicine (London, England)·2026
Same journal

Authors' response to Dr Lucy Williams' letter to the Editor: 'Life expectancy of UK physicians in the early 21st century: An analysis of 1,000 fellows from the Royal College of Physicians' Munk's Roll'.

Clinical medicine (London, England)·2026
Same journal

Resident doctor workforce wellbeing worldwide: lessons between the United Kingdom and Australia.

Clinical medicine (London, England)·2026
Same journal

Premature ventricular complexes.

Clinical medicine (London, England)·2026
See all related articles

Related Experiment Video

Updated: Aug 12, 2025

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

24.5K

Next-generation sequencing and molecular therapy.

Cienne Morton1, Debashis Sarker2, Paul Ross3

  • 1Medical Oncology Department, Guy's and St Thomas' NHS Foundation Trust, London, UK Cienne.morton@gstt.nhs.uk.

Clinical Medicine (London, England)
|January 25, 2023
PubMed
Summary
This summary is machine-generated.

Identifying critical cancer mutations is key. Next-generation sequencing aids in finding actionable biomarkers for targeted therapies, advancing personalized cancer medicine.

Keywords:
cancer genomicsliquid biopsynextgeneration sequencingtargeted therapy

More Related Videos

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

11.9K
Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
11:02

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

19.5K

Related Experiment Videos

Last Updated: Aug 12, 2025

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

24.5K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

11.9K
Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
11:02

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

19.5K

Area of Science:

  • Oncology
  • Genomics
  • Molecular Biology

Background:

  • Cancers possess numerous mutations, but only a few drive malignancy.
  • Understanding cancer's genomic complexity reveals actionable biomarkers.
  • Targeted therapies and immunotherapy show success in cancers like NSCLC, breast, and melanoma.

Purpose of the Study:

  • To highlight the role of next-generation sequencing in identifying actionable cancer mutations.
  • To discuss the shift towards targeted therapies and immunotherapy in cancer treatment.
  • To outline challenges in achieving personalized medicine for more cancer patients.

Main Methods:

  • Utilizing next-generation sequencing for high-throughput genomic interrogation.
  • Analyzing genomic data to detect actionable mutations and fusions.
  • Reviewing advancements in targeted therapy and immunotherapy development.

Main Results:

  • Next-generation sequencing enables efficient and affordable genomic analysis.
  • Detection of actionable mutations and fusions is facilitated for individual patients.
  • Identification of novel predictive and prognostic biomarkers is enhanced.

Conclusions:

  • Personalized medicine relies on identifying critical cancer mutations and developing targeted therapies.
  • Next-generation sequencing is crucial for advancing personalized cancer care.
  • Overcoming challenges in biopsy, sequencing, interpretation, and therapy development is essential for broader personalized medicine application.