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Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET

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Controlled ultrafast ππ-πσ dynamics in tryptophan-based peptides with tailored micro-environment.

Marius Hervé¹, Alexie Boyer¹, Richard Brédy¹

¹Univ Lyon, Université Claude Bernard Lyon 1, CNRS, Institut Lumière Matière, Villeurbanne, France.

Communications Chemistry

|January 25, 2023

Summary

Scientists controlled ultrafast molecular dynamics by altering the molecular environment. Changing a single atom in a charged adduct significantly extended the timescale of photo-induced processes in tryptophan.

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Area of Science:

Physical Chemistry
Molecular Dynamics
Spectroscopy

Background:

Ultrafast molecular dynamics are governed by potential energy surfaces and environmental interactions.
Understanding microscopic dynamics is challenging due to complex interactions.
Controlling molecular processes requires precise manipulation of their environment.

Purpose of the Study:

To investigate ultrafast photo-induced ππ*-πσ* dynamics in isolated tryptophan ions.
To explore the influence of a controlled micro-environment on molecular dynamics.
To determine how charged adducts affect charge and energy transfer timescales.

Main Methods:

Utilized electrospray and mass spectrometry to generate isolated molecular ions.
Introduced a single, charged adduct to tryptophan ions.
Measured ultrafast photo-induced dynamics using advanced spectroscopic techniques.

Main Results:

Observed a significant increase (over one order of magnitude) in dynamics timescale.
Demonstrated that changing the adduct atom drastically alters the timescale.
Proposed a model explaining adduct effects on electronic structure and dynamics.

Conclusions:

The molecular micro-environment can be engineered to control ultrafast dynamics.
Localized and delocalized electronic effects of adducts are key to controlling timescales.
Angström-scale design of the micro-environment offers new avenues for controlling molecular processes.