Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

127
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
127

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A uniform tissue-clearing framework and mesoSPIM-ultra enable cm-scale single-neuron tracing.

bioRxiv : the preprint server for biology·2026
Same author

Altair-dvOPM: an open-access platform for large-field three-dimensional tissue imaging.

bioRxiv : the preprint server for biology·2026
Same author

A deep-learning framework reveals whole-body perturbations at cell level.

Nature·2026
Same author

Lipid@polymer hybrid nanoparticles for efficient siRNA transport across the lung barriers: Mechanistic insights into the role of Ionizable lipids.

Journal of colloid and interface science·2026
Same author

Inhibition of ceramide synthesis ameliorates body wasting in a cancer cachexia model.

The Journal of clinical investigation·2026
Same author

Whole-mouse immunolabeling at cellular resolution for comprehensive 3D atlases.

Nature protocols·2026

Related Experiment Video

Updated: Aug 12, 2025

Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies
08:49

Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies

Published on: May 23, 2025

273

Whole-mouse clearing and imaging at the cellular level with vDISCO.

Ruiyao Cai1,2, Zeynep Ilgin Kolabas1,2,3, Chenchen Pan1,2

  • 1Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Munich, Munich, Germany.

Nature Protocols
|January 25, 2023
PubMed
Summary

We developed nanobody-boosted 3D imaging of solvent-cleared organs (vDISCO) to make whole mice transparent for cellular-resolution imaging. This method enhances and stabilizes fluorescence, enabling comprehensive whole-body analysis.

More Related Videos

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO
07:49

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO

Published on: July 7, 2014

26.8K
Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
08:49

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy

Published on: August 1, 2022

3.7K

Related Experiment Videos

Last Updated: Aug 12, 2025

Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies
08:49

Rapid Whole-Mount High-Resolution Imaging of Small Animal Vasculature for Quantitative Studies

Published on: May 23, 2025

273
Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO
07:49

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO

Published on: July 7, 2014

26.8K
Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
08:49

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy

Published on: August 1, 2022

3.7K

Area of Science:

  • Biology
  • Biotechnology
  • Microscopy

Background:

  • Homeostatic and pathological processes impact multiple organs systemically.
  • Advances in tissue clearing and microscopy enable holistic biological sample examination.

Purpose of the Study:

  • To present a detailed protocol for nanobody-boosted 3D imaging of solvent-cleared organs (vDISCO).
  • To enable whole-body imaging at cellular resolution for comprehensive biological analysis.

Main Methods:

  • vDISCO is a pressure-driven, nanobody-based whole-body immunolabeling and clearing technique.
  • The protocol renders whole mice transparent within 3 weeks, enhancing and stabilizing fluorescent signals.
  • The method is accessible to labs with limited tissue clearing expertise.

Main Results:

  • vDISCO significantly boosts fluorescence signal from genetically expressed reporters.
  • The method allows reliable detection and quantification of fluorescent signals in intact rodents.
  • Applications include whole-mouse neuronal mapping, metastasis detection, lymphatic system visualization, virus tracing, and brain pericyte imaging.

Conclusions:

  • vDISCO provides a versatile pipeline for systematic, comprehensive studies of cellular phenomena and connectivity in whole bodies.
  • This technique facilitates the analysis of entire organisms at cellular resolution.