A Novel Electrochemical Biosensor Based on Polyaniline-Embedded Copper Oxide Nanoparticles for High-Sensitive...

This paper proposes a fabrication of a hyper-sensitive amperometric biosensor for paraoxon-ethyl (PE) detection. In this developed biosensor, polyaniline (PANI) and copper oxide (CuO)-based nanocomposite is used as a sensing platform. The homogeneous distribution of CuO onto the PANI matrix enhances the surface area and conductivity of the nanocomposite. Additionally, the PANI produces a compatible environment for enzyme immobilization, which further enhances the rate of electron transfer. For biosensor fabrication, the nanocomposite is deposited electrophoretically onto the ITO glass substrate and immobilization of acetylcholinesterase (AChE) enzyme is conducted onto the fabricated electrode surface. The results validate good reproducibility, good stability, and high selectivity of the fabricated biosensor (AChE/PANI@CuO/ITO). The inhibition rate of paraoxon-ethyl (PE) is recorded in the concentration range of 1-200 nM with a low limit of detection of 0.096 nM or 96 pM. The sensitivity of the developed biosensor is found to be 49.86 µA(nM)^-1. The developed biosensor is further successfully accomplished for the detection of PE in real samples like rice and pulse.