Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Gene Expression?01:36

What is Gene Expression?

8.9K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
8.9K
Alternative RNA Splicing02:18

Alternative RNA Splicing

21.6K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.6K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.1K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.1K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

969
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
969
MicroRNAs01:22

MicroRNAs

3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
Types of RNA01:20

Types of RNA

6.0K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reply to: "Hypoxia-Induced Lineage Plasticity in Neuroblastoma: Advancing Signature Interpretation and Clinical Translation".

Clinical and translational science·2026
Same author

Multi-omics and developmental comparison of direct and conventional warming methods in vitrified human and mouse cleavage-stage embryos.

Human reproduction (Oxford, England)·2026
Same author

Maternal trans-vaccenic acid shapes neonatal T cell development and early-life immune imprinting.

Science (New York, N.Y.)·2026
Same author

Spatially resolved m<sup>6</sup>A profiling using m<sup>6</sup>A-ARTR-DBiT.

Nature methods·2026
Same author

Gut Microbiota-derived Acetate Safeguards the Colonic Epithelial Acetyl-CoA Reserve to Avert Colonic Senescence.

bioRxiv : the preprint server for biology·2026
Same author

Cryptic splicing in synaptic and membrane excitability genes links TDP-43 loss to neuronal dysfunction.

Science translational medicine·2026

Related Experiment Video

Updated: Aug 12, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.0K

Exon architecture controls mRNA m6A suppression and gene expression.

P Cody He1,2,3, Jiangbo Wei1,3, Xiaoyang Dou1,3

  • 1Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637, USA.

Science (New York, N.Y.)
|January 27, 2023
PubMed
Summary

Exon junction complexes (EJCs) suppress N-methyladenosine (m6A) deposition on messenger RNA (mRNA), regulating gene expression. This suppression impacts mRNA stability and is influenced by exon architecture.

More Related Videos

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
08:58

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

Published on: May 1, 2019

15.1K
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.0K

Related Experiment Videos

Last Updated: Aug 12, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.0K
In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
08:58

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

Published on: May 1, 2019

15.1K
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.0K

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Epigenetics

Background:

  • N-methyladenosine (m6A) is the most prevalent mRNA modification, crucial for various cellular functions.
  • The precise regulation of m6A deposition across the transcriptome is not fully understood.

Purpose of the Study:

  • To investigate the global regulators of m6A specificity.
  • To identify factors that prevent m6A deposition in specific transcriptome regions.

Main Methods:

  • Utilized a massively parallel assay for m6A (MPm6A) to map m6A modification sites genome-wide.
  • Investigated the role of exon junction complexes (EJCs) in m6A regulation.

Main Results:

  • Discovered that EJCs act as global suppressors of m6A deposition.
  • EJCs protect exon junction-proximal RNA within coding sequences from methylation.
  • EJC-mediated m6A suppression influences mRNA stability and is dependent on exon length and position.
  • EJC-suppressed methylation sites correlate with EJC-suppressed splice sites, indicating exon architecture's role in RNA accessibility.

Conclusions:

  • EJCs are key regulators of m6A specificity, preventing methylation in specific RNA regions.
  • Exon architecture plays a significant role in determining the accessibility of mRNA to regulatory complexes like EJCs.
  • EJC-mediated m6A suppression is a critical mechanism for controlling mRNA fate and function.