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The development of microfluidic-based western blotting: Technical advances and future perspectives
Christopher T Desire1, R Dario Arrua1, Xanthe L Strudwick1
1Future Industries Institute, University of South Australia, GPO Box 2471, Adelaide, SA 5001, Australia.
Journal of Chromatography. A
|January 29, 2023
Summary
Microfluidic technologies have significantly advanced western blotting over 20 years, improving single-cell analysis and quantitation. Future developments may enable point-of-care diagnostic devices.
Area of Science:
- Biotechnology and Biomedical Engineering
- Analytical Chemistry
Background:
- Western blotting is a fundamental technique in molecular biology for protein detection.
- Traditional western blotting methods face challenges in speed, sensitivity, and automation.
Approach:
- This review critically examines the integration of microfluidic technologies into western blotting.
- It analyzes advancements in microfluidic western blotting systems, including commercially available platforms.
- The review covers novel approaches like projection electrophoresis and triBlots.
Key Points:
- Microfluidics offers advantages such as reduced sample/reagent consumption and faster assay times.
- Developments include systems for single-cell western blotting and enhanced quantitation algorithms.
- Exploration of new materials and chemistries, alongside projection electrophoresis, are key innovations.
Conclusions:
- Microfluidic western blotting has matured significantly, with commercially available solutions emerging.
- Future directions point towards increased automation, improved quantitation, and potential point-of-care applications.
- These advancements promise to make western blotting more accessible and efficient in clinical settings.
