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Updated: Aug 12, 2025

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

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Astrocyte and neuronal Panx1 support long-term reference memory in mice.

Price Obot¹, Galadu Subah¹, Antonia Schonwald¹

¹Department of Cell Biology and Anatomy, New York Medical College, Valhalla, NY, 10595.

Biorxiv : the Preprint Server for Biology

|January 30, 2023

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Pannexin 1 (Panx1) channels are crucial for long-term spatial memory consolidation in mice. Both neuronal and astrocyte Panx1 are essential for this process, impacting hippocampus-dependent learning.

Area of Science:

Neuroscience
Molecular Biology
Physiology

Background:

Pannexin 1 (Panx1) proteins form plasma membrane channels involved in cell signaling.
Panx1 channel activation is linked to neurological disorders, but its physiological role in learning is understudied.
Panx1 channels may mediate neuron-glia interactions critical for brain function.

Approach:

Utilized Panx1 transgenic mice with global and cell-type specific deletions.
Assessed working and long-term spatial reference memory using the 8-arm radial maze.
Performed field potential recordings in hippocampal slices to analyze synaptic plasticity.

Key Points:

Panx1-null mice exhibit deficits in long-term spatial reference memory, but not working memory.
Both astrocyte and neuronal Panx1 are necessary for consolidating long-term spatial memory.

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Related Experiment Videos

Last Updated: Aug 12, 2025

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

Published on: July 7, 2022

Isolation and Culture of Mouse Cortical Astrocytes

Isolation and Culture of Mouse Cortical Astrocytes

Published on: January 19, 2013

Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice

Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice

Published on: June 22, 2021

Hippocampal slices from Panx1-null mice show attenuated long-term potentiation (LTP) and long-term depression (LTD).

Conclusions:

Neuronal and astrocyte Panx1 channels are critical for the development and maintenance of long-term spatial reference memory.
Panx1 channels play a significant physiological role in hippocampus-dependent learning and synaptic plasticity.
These findings highlight Panx1 as a potential target for cognitive disorders.