Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
Tomoharu Kanie1,2, Roy Ng1, Keene L Abbott1
1Baxter Laboratory, Department of Microbiology & Immunology and Department of Pathology, Stanford University, Stanford, CA, 94305.
Biorxiv : the Preprint Server for Biology
|January 30, 2023
Summary
Researchers identified Neuronal Calcium Sensor-1 (NCS1) as key to recruiting ciliary vesicles to the mother centriole
Area of Science:
- Cell Biology
- Molecular Biology
- Organelle Biology
Background:
- Primary cilia are essential microtubule-based organelles involved in various cellular functions.
- Cilium formation requires the recruitment of ciliary vesicles to the mother centriole's distal appendage.
- The precise mechanism of ciliary vesicle capture by the distal appendage remained unknown.
Conclusions:
- CEP89 recruits NCS1 to the distal appendage, initiating the capture of ciliary vesicles.
- NCS1, via its myristoylation motif, directly mediates the recruitment of ciliary vesicles to the distal appendage.
- This study reveals the first described mechanism for distal appendage-mediated ciliary vesicle recruitment, a critical step in primary cilium biogenesis.
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