Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Aug 12, 2025

The Floating Lab: Standard Operational Procedure for Collecting and Filtering Seawater Samples from Operating Ferries for Environmental DNA Analysis
06:22

The Floating Lab: Standard Operational Procedure for Collecting and Filtering Seawater Samples from Operating Ferries for Environmental DNA Analysis

Published on: August 1, 2025

195

Capturing marine microbiomes and environmental DNA: A field sampling guide.

Nastassia Virginia Patin1,2,3, Kelly D Goodwin1,3

  • 1Atlantic Oceanographic and Meteorological Laboratory, Ocean Chemistry and Ecosystems Division, National Oceanic and Atmospheric Administration, Miami, FL, United States.

Frontiers in Microbiology
|January 30, 2023
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Underway measurement of cyanobacterial microcystins using a surface plasmon resonance sensor on an autonomous underwater vehicle.

Limnology and oceanography, methods·2025
Same author

Critical considerations for communicating environmental DNA science.

Environmental DNA (Hoboken, N.J.)·2024
Same author

The founding charter of the Omic Biodiversity Observation Network (Omic BON).

GigaScience·2023
Same author

Editorial: Marine microbiomes: towards standard methods and Best Practices.

Frontiers in microbiology·2023
Same author

A Preliminary Investigation of Fecal Indicator Bacteria, Human Pathogens, and Source Tracking Markers in Beach Water and Sand.

Environment research journal·2022
Same author

Tourmaline: A containerized workflow for rapid and iterable amplicon sequence analysis using QIIME 2 and Snakemake.

GigaScience·2022

Standardizing marine microbiome and environmental DNA (eDNA) sample collection and preservation is crucial for comparative studies. This work provides guidelines for fit-for-purpose protocols, emphasizing freezing over buffer preservation for better results.

Area of Science:

  • Marine biology
  • Environmental science
  • Molecular ecology

Background:

  • Growing interest in marine microbiome and environmental DNA (eDNA) necessitates standardized sampling and preservation methods.
  • Current practices vary widely, hindering cross-study comparisons and meta-analyses.
  • Lack of standardized protocols limits the reliability and comparability of marine microbiome and eDNA data.

Purpose of the Study:

  • To provide guidelines for marine microbiome and eDNA sample collection and preservation.
  • To enable the design of fit-for-purpose protocols tailored to specific research goals.
  • To support comparative assessments and meta-analyses of marine microbiome and eDNA data.

Main Methods:

  • Review of published methods and field sampling experiences.
Keywords:
eDNAfield methodsmicrobiomemolecular ecological methodssampling

More Related Videos

Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
10:43

Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology

Published on: November 5, 2014

25.8K
Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands
07:26

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands

Published on: January 31, 2025

400

Related Experiment Videos

Last Updated: Aug 12, 2025

The Floating Lab: Standard Operational Procedure for Collecting and Filtering Seawater Samples from Operating Ferries for Environmental DNA Analysis
06:22

The Floating Lab: Standard Operational Procedure for Collecting and Filtering Seawater Samples from Operating Ferries for Environmental DNA Analysis

Published on: August 1, 2025

195
Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
10:43

Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology

Published on: November 5, 2014

25.8K
Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands
07:26

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands

Published on: January 31, 2025

400
  • Consideration of environmental factors, resource availability, and experimental objectives.
  • Focus on sample processing, storage, and eDNA extraction protocols for prokaryotic and microeukaryotic eDNA.

    • Main Results:

    • Development of guidelines for designing fit-for-purpose sampling protocols.
    • Recommendations include rigorous decontamination, pilot studies for filtration volume, and prioritizing sample freezing.
    • An annotated list of relevant studies and a sample protocol for oceanographic vessels are provided.

    Conclusions:

    • Standardized, fit-for-purpose protocols are essential for robust marine microbiome and eDNA research.
    • Prioritizing sample freezing and conducting pilot studies are key recommendations.
    • These guidelines will aid scientists in making informed decisions for marine eDNA and microbiome sampling.