Diaminoanthraquinone Enhances Alkaloid Ionization in MALDI-MS
Tohru Yamagaki1, Tsukiho Osawa1
1Bioorganic Research Institute, Suntory Foundation for Life Sciences, 8-1-1 Seikadai, Seika-cho, Soraku-gun, Kyoto 619-0284, Japan.
Abstract:
The alkaloids epinastine, 3-methylxanthine and camptothecin were analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The ionization efficiencies of epinastine and 3-methylxanthine were improved upon the addition of 1,5-diaminoanthraquinone (DAAQ). DAAQ did not show ultraviolet absorbance peaks at wavelengths around 337 nm and 355 nm that are used in conventional MALDI-MS instruments. In addition, the DAAQ ion peak was very weak relative to those of the analytes due to the low absorbance efficiency. These properties of DAAQ are advantageous for the DAAQ-MALDI-MS analysis of alkaloids.
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