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Updated: Aug 12, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
Published on: June 16, 2017
Rapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC
Sameer Singh1,2, Anoosha Banerjee1, Arnaud Vanden Broeck1
1Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY, 10065, USA.
Abstract:
One of the challenges faced by current CRISPR/Cas9 editing strategies is the difficulty in rapidly selecting clonal populations of biallelically edited cells. Here we present Surface engiNeered fluorEscence Assisted Kit with Protein Epitope Enhanced Capture (SNEAK PEEC), a platform that combines human genome editing with cell-surface display, which enables the direct identification of biallelically edited clones with minimal screening.
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