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Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Published on: November 30, 2016
Expression analysis of miRNA-155 level in Helicobacter pylori related inflammation and chronic gastritis
Ramina Mahboobi1, Fatemeh Fallah1,2, Abbas Yadegar3
1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Background And Objectives:
Helicobacter pylori, is a major etiologic agent associated with gastritis. There is more evidence of noncoding microRNAs (miRs) dysregulation in gastrointestinal diseases, including inflammation caused by Helicobacter pylori. Also, the classification of gastrointestinal malignancies using the miRs profile is better than the protein profile. MiRNA-155(miRNA-155) among other miRs plays an important role in control of inflammation and gastric malignancy, so it can be remarkable prognosis marker of gastric cancer in the phase of chronic gastritis. The aim of this study was to compare the expression of miRNA-155 in gastric biopsy and serum samples of adult patients with chronic gastritis.
Materials And Methods:
Biopsy and blood samples were collected from endoscopy candidates at Taleghani hospital, Tehran, during 2019. H. pylori infection was detected using histology, culture and molecular PCR methods. Based on cagA and vacA genotyping, the toxicity of H. pylori isolates were determined. After RNA extraction, the expression rate of miRNA-155 was evaluated by real-time polymerase chain reaction (RT-PCR) in gastric tissue and serum of adults infected by H. pylori (n = 30) compared with control group without infection (n = 20). RNU6 housekeeping miRNA were used as endogenous control and statistical analyses were performed using SPSS, ANOVA and Student's t-test.
Results:
miRNA-155 expression in H. pylori infected adult patients increased significantly by 5.61 and 10.11 fold in serum and tissue respectively, compared to that observed in the control group. Evaluation of miRNA-155 expression pattern in relation to bacterial virulence factors showed that the increase in miRNA-155 expression is independent of CagA and VacA toxins.
Conclusion:
According to the differential expression patterns of miRNA-155 in serum samples of the infected adult patients, miRNA-155 has the potential to evaluate as chronic gastritis marker.
Insights
MicroRNA-155 (miRNA-155) expression significantly increases in the serum and gastric tissue of adults with chronic gastritis due to Helicobacter pylori infection. This elevated miRNA-155 shows potential as a diagnostic marker for gastritis.
Area of Science:
- Gastroenterology
- Molecular Biology
- Oncology
Background:
- Helicobacter pylori infection is a primary cause of gastritis and is linked to gastrointestinal diseases.
- Noncoding microRNAs (miRNAs) are increasingly recognized for their role in inflammation and malignancy.
- MiRNA-155 is implicated in inflammation control and gastric cancer development.
Purpose of the Study:
- To compare miRNA-155 expression in gastric biopsy and serum samples of adult patients with chronic gastritis.
- To investigate the potential of miRNA-155 as a prognostic marker for gastric cancer in chronic gastritis.
- To assess the relationship between miRNA-155 expression and H. pylori virulence factors.
Main Methods:
- Collected gastric biopsy and blood samples from H. pylori-infected adults and a control group.
- Detected H. pylori infection using histology, culture, and PCR, with genotyping for CagA and VacA.
- Quantified miRNA-155 expression using real-time RT-PCR in tissue and serum samples.
Main Results:
- Significantly higher miRNA-155 expression (5.61-fold in serum, 10.11-fold in tissue) was observed in H. pylori-infected patients compared to controls.
- The increased miRNA-155 expression was independent of CagA and VacA toxins.
- Differential expression patterns of miRNA-155 were noted in serum samples.
Conclusions:
- MiRNA-155 expression is significantly elevated in adult patients with H. pylori-induced chronic gastritis.
- Serum miRNA-155 demonstrates potential as a reliable biomarker for diagnosing chronic gastritis.
- Further research may validate miRNA-155 as a prognostic indicator for gastric cancer.
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