Comprehensive Analysis of RNA-Binding Protein-Related lncRNA in Breast Invasive Carcinoma

Area of Science:

• Oncology
• Molecular Biology
• Bioinformatics

Background:

• RNA-binding proteins (RBPs) regulate RNA function and stability in cancers like breast invasive carcinoma (BRCA).
• The roles of RBP-related long noncoding RNAs (lncRNAs) in BRCA remain largely unexplored.
• Understanding these interactions is crucial for advancing BRCA treatment strategies.

Purpose of the Study:

• To identify and characterize RBP-related lncRNAs in BRCA.
• To develop and validate a prognostic signature (RBPLSig) for BRCA patients.
• To explore the clinical utility of RBPLSig in predicting patient outcomes and immune microenvironment.

Main Methods:

• Bioinformatic analysis to identify RBP-related lncRNAs and construct the RBPLSig.
• Development of an online tool for survival rate prediction.
• Experimental validation of lncRNA WAC-AS1 function using qRT-PCR, knockdown, CCK-8, and TUNEL assays.
• Molecular docking to investigate potential mechanisms of RBP gene NSUN6.

Main Results:

• The developed RBPLSig serves as an independent prognostic factor for BRCA patients.
• RBPLSig is implicated in malignancy-associated immunoregulatory pathways.
• Distinct immune statuses and therapeutic responses were observed between high- and low-risk groups stratified by RBPLSig.

Conclusions:

• This study enhances the understanding of RBP-related lncRNAs in BRCA.
• A novel, independent RBPLSig is demonstrated for prognostic assessment and immune microenvironment evaluation.
• The findings can guide personalized treatment decisions for BRCA patients.