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Gold-based paper for antigen detection of monkeypox virus
Liya Ye1,2, Xianlu Lei1,2, Xinxin Xu1,2
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China. xxx89826@163.com.
The Analyst
|February 1, 2023
Summary
A new immunochromatographic test strip enables rapid monkeypox virus diagnosis. This assay detects the monkeypox virus A29 protein, offering a sensitive tool for early detection and control of the viral disease.
Area of Science:
- Virology
- Immunology
- Biotechnology
Background:
- The 2022 monkeypox virus outbreak highlighted the need for rapid diagnostic tools.
- Early diagnosis is critical for controlling the spread of atypical outbreaks.
Purpose of the Study:
- To develop a rapid and sensitive assay for monkeypox virus early diagnosis.
- To establish an immunochromatographic test strip for detecting the monkeypox virus A29 protein.
Main Methods:
- Expression of monkeypox virus A29 protein and homologous viral proteins in E. coli.
- Generation of 25 monoclonal antibodies against the A29 protein using hybridoma technology.
- Development of an immunochromatographic test strip assay utilizing specific monoclonal antibodies.
Main Results:
- Monoclonal antibodies mAb-7C5 and 5D8 demonstrated high sensitivity for A29 detection.
- The assay achieved a low limit of detection of 50 pg/mL for purified A29.
- The test strips showed high specificity, with no cross-reactivity to other orthopoxviruses or common respiratory pathogens.
Conclusions:
- The developed immunochromatographic test strip is a viable method for early and rapid monkeypox virus diagnosis.
- Antigen detection via this assay aids in effective outbreak prevention and control efforts.
