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Author Spotlight: Investigating Cellular and Molecular Dynamics During Muscle Regeneration Using Cutting-Edge Single-Cell Technologies
Published on: December 1, 2023
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Three-dimensional chromatin re-organization during muscle stem cell aging.
Benjamin A Yang1,2, Jacqueline A Larouche1,2, Kaitlyn M Sabin1,2
1Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Aging Cell
|February 2, 2023
Summary
Stem cell aging causes skeletal muscle atrophy. Genome 3D structure changes locally, impacting gene expression and function in aging muscle stem cells.
Area of Science:
- Gerontology
- Molecular Biology
- Genomics
Background:
- Sarcopenia, age-related muscle loss, is a growing health concern.
- Muscle stem cells are crucial for muscle repair but decline with age.
- Genome organization influences stem cell aging mechanisms.
Purpose of the Study:
- To investigate changes in 3D genome architecture during muscle stem cell aging.
- To correlate chromatin structure alterations with gene expression changes.
Main Methods:
- Generated 3D chromatin conformation maps (Hi-C).
- Integrated Hi-C data with chromatin accessibility and transcriptome profiles.
- Analyzed bulk and single-cell data from young and old muscle stem cells.
Main Results:
- Global chromatin organization remained stable in aging muscle stem cells.
- Local chromatin contacts were extensively rewired at finer scales.
- Rewiring correlated with altered transcription factor binding and gene expression.
Conclusions:
- Aging muscle stem cells exhibit specific changes in 3D genome topology.
- Genome organization plays a key role in regulating molecular function during stem cell aging.
- Findings offer insights into the mechanisms of sarcopenia.
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