Comparative study by immunofluorescence of T and P antigens induced by adenovirus type 12 in permissive and nonpermissive cells
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The development of adenovirus type 12 T antigen and of the complex of antigenic early proteins designated as P antigen was studied by immunofluorescence in productively infected KB cells and abortively infected RK-13 cells. T antigen is detected in both cell types very early in infection. In KB cells it presents the well known pattern of nuclear dots and flecks but in RK-13 cells at the time of maximum abundance, 18 hours p.i., T antigen forms a net of long filaments that fills the nucleus. Later, part of the filaments condense into a large aggregate that finally is apparently degraded. P antigen in infected RK-13 cells looks like T antigen in KB cells. In these cells, besides an early phase wherein P antigen is almost indistinguishable from T antigen, a late component is evident under the form of large balls and rosettes. The possible identification of this component with the DNA binding protein is discussed.
The development of adenovirus type 12 T antigen and of the complex of antigenic early proteins designated as P antigen was studied by immunofluorescence in productively infected KB cells and abortively infected RK-13 cells. T antigen is detected in both cell types very early in infection. In KB cells it presents the well known pattern of nuclear dots and flecks but in RK-13 cells at the time of maximum abundance, 18 hours p.i., T antigen forms a net of long filaments that fills the nucleus. Later, part of the filaments condense into a large aggregate that finally is apparently degraded. P antigen in infected RK-13 cells looks like T antigen in KB cells. In these cells, besides an early phase wherein P antigen is almost indistinguishable from T antigen, a late component is evident under the form of large balls and rosettes. The possible identification of this component with the DNA binding protein is discussed.