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LIP5, a MVB biogenesis regulator, is required for rice growth
Mengxue Wang1, Shuwei Luo1, Baofang Fan2
1College of Life Science and Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province, China Jiliang University, Hangzhou, China.
Frontiers in Plant Science
|February 3, 2023
Summary
Rice OsLIP5 complements Arabidopsis atlip5 mutants, restoring disease resistance and salt tolerance. Unlike in Arabidopsis, OsLIP5 is crucial for rice growth and development, highlighting evolutionary divergence in LIP5 function.
Area of Science:
- Plant Biology
- Molecular Biology
- Genetics
Background:
- LYST-INTERACTING PROTEIN5 (LIP5) regulates multivesicular body (MVB) biogenesis in eukaryotes.
- In Arabidopsis, AtLIP5 mediates stress-induced MVB biogenesis, but atlip5 mutants show normal growth.
- AtLIP5 is targeted by stress-responsive MITOGEN-ACTIVATED PROTEIN KINASE3 and 6.
Purpose of the Study:
- To investigate the function of rice OsLIP5 in plant growth, stress responses, and MVB biogenesis.
- To compare the role of LIP5 in rice versus Arabidopsis.
Main Methods:
- Complementation analysis using rice OsLIP5 in Arabidopsis atlip5 mutants.
- Phenotypic characterization of rice oslip5 mutants.
- Analysis of endocytosis regulation by LIP5 in both species.
Main Results:
- Rice OsLIP5 fully restored disease resistance and salt tolerance in Arabidopsis atlip5 mutants.
- Rice oslip5 mutants exhibited severe growth defects, necrosis, and premature death.
- LIP5 regulates endocytosis in rice under both normal and stress conditions, unlike in Arabidopsis.
Conclusions:
- OsLIP5 plays a critical role in normal plant growth and stress responses in rice.
- There is significant evolutionary divergence in the function of the conserved LIP5-regulated MVB pathway across plant species.
- LIP5's role in endocytosis differs between rice and Arabidopsis, impacting normal development.
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