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Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
Published on: March 9, 2012
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RCAS1 increases cell morphological changes in murine fibroblasts by reducing p38 phosphorylation
Takuya Nishinakagawa1, Mai Hazekawa1, Masato Hosokawa1
1Department of Immunological and Molecular Pharmacology, Faculty of Pharmaceutical Science, Fukuoka University, Fukuoka 814‑0180, Japan.
Molecular Medicine Reports
|February 3, 2023
Summary
Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) inhibits tumor cell growth and promotes apoptosis by affecting actin cytoskeleton dynamics. RCAS1 overexpression reduces actin stress fibers and p38 phosphorylation, impacting cell morphology and survival.
Area of Science:
- Oncology
- Immunology
- Cell Biology
Background:
- Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a tumor antigen implicated in various human malignancies.
- RCAS1 expression correlates with advanced tumor characteristics and poor patient survival, suggesting a role in immune evasion.
- Previous studies demonstrated RCAS1 overexpression inhibits cell cycle progression and induces apoptosis.
Purpose of the Study:
- To investigate the signaling pathways responsible for RCAS1-induced changes in cell morphology.
- To elucidate the molecular mechanisms underlying RCAS1's impact on the actin cytoskeleton.
Main Methods:
- Utilized doxycycline-inducible RCAS1 overexpression in murine fibroblast L cells.
- Analyzed changes in actin stress fibers, cofilin phosphorylation, and p38 phosphorylation.
- Assessed cell morphology and apoptosis induction.
Main Results:
- Increased RCAS1 expression led to reduced actin stress fibers and decreased cofilin phosphorylation.
- RCAS1 overexpression significantly decreased p38 phosphorylation.
- These cytoskeletal changes preceded caspase-mediated apoptosis.
Conclusions:
- RCAS1 influences cell morphology and apoptosis through modulation of actin dynamics and the p38 signaling pathway.
- Understanding these pathways could reveal new therapeutic targets for RCAS1-expressing cancers.
Keywords:
actin dynamicscell morphological changep38 MAP kinasereceptor‑binding cancer antigen expressed on SiSo cellsMore Related Videos
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