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Injectable Double-Network Hydrogel-Based Three-Dimensional Cell Culture Systems for Regenerating Dental Pulp.

Bing Han¹, Chunling Cao¹, Aijing Wang¹

¹Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing 100081, China.

ACS Applied Materials & Interfaces

|February 3, 2023

Summary

Injectable double-network hydrogels show promise for dental pulp regeneration. These glycol chitosan-based scaffolds offer improved mechanical properties and degradation profiles, supporting human dental pulp stem cell differentiation for effective tissue repair.

Keywords:

double network human dental pulp stem cells injectable hydrogel matrix stiffness pulp regeneration

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Author Spotlight: Advancing Tissue Regeneration and Disease Modeling with Dental Pulp Stem Cells

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Published on: May 5, 2023

Area of Science:

Biomaterials Science
Regenerative Medicine
Dental Engineering

Background:

Dental pulp regeneration is crucial but challenging in dentistry.
Hydrogel scaffolds are promising for simulating extracellular matrix and guiding tissue regeneration.
Optimizing biocompatibility, mechanical properties, and degradation is essential for effective pulp regeneration.

Purpose of the Study:

To develop injectable double-network (DN) hydrogel systems for dental pulp regeneration.
To compare the properties of DN hydrogels with single-network (SN) hydrogels.
To evaluate the in vitro and in vivo biological performance of DN hydrogels with encapsulated human dental pulp stem cells (hDPSCs).

Main Methods:

Development of injectable double-network (DN) and single-network (SN) glycol chitosan-based hydrogels.
Characterization of hydrogel microstructure, mechanical properties, rheology, and degradation in a simulated root canal.
Encapsulation of hDPSCs and evaluation of in vitro (odontogenic differentiation, mineralization) and in vivo (tissue integration, Smad3 activation) performance.

Main Results:

DN hydrogels exhibited ideal injectability under physiological conditions due to dynamic crosslinks.
DN hydrogels demonstrated superior mechanical properties and longer degradation duration compared to SN hydrogels.
The DN hydrogel system promoted odontogenic differentiation and mineralization of hDPSCs in vitro and showed favorable in vivo tissue integration.

Conclusions:

Injectable DN glycol chitosan-based hydrogels are suitable for dental pulp regeneration.
The properties of DN hydrogels mimic pulp-like connective tissue, potentially via Smad3 activation.
These findings support the clinical application of DN hydrogels for regenerating dental pulp tissue.