Anti-biofilm effects and healing promotion by silver oxynitrate-based dressings

Microbial growth within a wound often manifests as biofilms, which can prevent healing and is difficult to eradicate. Novel silver dressings claim to combat wound infection, but anti-biofilm efficacy and effects on healing independent of infection are often unclear. Using in vitro and in vivo S. aureus and P. aeruginosa biofilm models, we report the efficacy of a dressing which produces Ag¹⁺ ions; an Ag¹⁺ dressing containing ethylenediaminetetraacetic acid and benzethonium chloride (Ag¹⁺/EDTA/BC), and a dressing containing silver oxynitrate (Ag Oxysalts) which produces Ag¹⁺, Ag²⁺ and Ag³⁺ ions, against wound biofilms, and their effects on healing. Ag¹⁺ dressings had minimal effect on in vitro and murine (C57BL/6j) wound biofilms. In contrast, Ag Oxysalts and Ag¹⁺/EDTA/BC dressings significantly reduced viable bacteria within in vitro biofilms and demonstrated a visible reduction in bacteria and EPS components within murine wound biofilms. The dressings had different effects on the healing of biofilm-infected and uninfected wounds, with Ag Oxysalts dressings having a greater beneficial effect on re-epithelialisation, wound size and inflammation than the control treatment and the other silver dressings. The different physicochemical properties of the silver dressings result in varied effects on wound biofilms and healing which should be considered when selecting dressings to treat biofilm-infected wounds.