Related Experiment Video
Updated: Aug 11, 2025
IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
Published on: July 7, 2015
Tryptophan capped gold-silver bimetallic nanoclusters-based turn-off fluorescence sensor for the determination of
S Swathy1, Goldamol S Pallam1, K Girish Kumar1
1Department of Applied Chemistry, Cochin University of Science and Technology, Kochi, 682022, Kerala, India.
Abstract:
Tryptophan capped gold-silver bimetallic nanoclusters (Trp-Au/Ag BNCs) based turn-off fluorescence sensor has been developed for the simple and rapid determination of histamine. Sensor parameters such as the effect of solvent, irradiation time etc. were optimized and further studies were done using these results. Fluorescence quenching of BNCs happens with the incremental addition of histamine. This might be due to the aggregation of BNCs induced by the electrostatic force of attraction between the negatively charged BNCs and amino functional groups of histamine. The sensor showed good sensitivity for the determination of histamine with a linear range of 8.5 × 10-5 M to 4.0 × 10-6 M and a limit of detection (LOD) of 9.0 × 10-7 M. Practical utility of the proposed sensor has been proved through spike recovery analysis in synthetic physiological fluids.
