Polyethyleneimine capped silver nanoclusters based turn-off-on fluorescence sensor for the determination of...

A polyethyleneimine capped silver nanoclusters (PEI-AgNCs) based turn-off-on fluorescence sensor has been developed to determine glutathione (GSH) effectively. The fluorescence intensity of silver nanoclusters (AgNCs) has been quenched by Cu(II) and recovered by adding GSH. The quenching of fluorescence intensity of PEI-AgNCs by Cu(II) and recovery of the emission intensity of PEI-AgNCs after the addition of GSH is supposed to be ground state adduct formation. Due to the greater affinity of Cu(II) towards GSH compared to that to PEI-AgNCs, the defragmentation of PEI-AgNCs-Cu(II) adduct occurs after the addition of GSH to the solution, resulting in the recovery of emission intensity of PEI-AgNCs. Characterisation studies of the probe have been done using FT-IR spectroscopy, XPS analysis, XRD analysis, UV-visible and Fluorescence spectrophotometry, EDX spectroscopy and TEM analysis. Different experimental parameters were optimised. Under optimised analytical conditions, the sensor showed a wide linear range for the quantification of GSH from 1.00 × 10^-4 M to 3.00 × 10^-6 M with a detection limit (LOD) of 8.00 × 10^-7 M. Selectivity and interference studies were done in the presence of different structurally similar and coexisting species of GSH in blood. The practical utility of the proposed sensor has been validated in artificial blood serum.