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Related Experiment Video

Updated: Aug 11, 2025

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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Thiophene-Extended Fluorescent Nucleosides as Molecular Rotor-Type Fluorogenic Sensors for Biomolecular Interactions.

Tomotaka Kumagai1, Ban Kinoshita1, Shingo Hirashima1

  • 1Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.

ACS Sensors
|February 6, 2023
PubMed
Summary

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Researchers developed novel fluorescent molecular rotors integrated into thymine nucleosides. These probes detect microenvironmental changes and protein interactions through vivid fluorescence, advancing biomolecular investigation.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • Fluorescent molecular rotors are crucial for studying biological systems.
  • Integrating these rotors with nucleic acids offers enhanced programmability.
  • Few molecular rotor-type isomorphic nucleosides have been developed.

Purpose of the Study:

  • To synthesize and characterize novel molecular rotor-containing nucleosides.
  • To develop sensitive fluorogenic sensors for biomolecular interactions and microenvironments.
  • To explore their potential as probes in biological systems.

Main Methods:

  • Synthesis of a thymine nucleoside-based molecular rotor.
  • Synthesis of a 2'-O-methyluridine analogue.
  • Characterization of the synthesized compounds and their fluorescence properties.
Keywords:
Z helixfluorescent nucleic acidsmolecular rotorprotein interactionviscosity response

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Last Updated: Aug 11, 2025

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Main Results:

  • A highly emissive molecular rotor-containing thymine nucleoside () was synthesized.
  • A 2'-O-methyluridine analogue (2'-OMe-) was also prepared.
  • Both compounds exhibit vivid fluorescence upon interaction with target proteins, indicating sensitivity to microenvironmental changes.

Conclusions:

  • The developed nucleoside-based molecular rotors are effective fluorogenic sensors.
  • These probes show potential for monitoring viscosity and protein-binding in biological systems.
  • They offer a promising platform for fluorescence mapping and biomolecular interaction studies.