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Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors
Published on: December 3, 2013
Column purification of adenovirus cores
J F Cupo1, L J Rezanka, J A Harpst
1Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Analytical Biochemistry
|July 1, 1987
Abstract:
A simple and efficient technique for purifying adenoviral chromatin (nucleoprotein cores) with Sephacryl S-1000 is described. This method is significantly faster than previous methods and gives a higher degree of purity with an increased recovery of the nucleoprotein. In addition, the structural integrity of the cores is maintained.
