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An azophenolic colorimetric reagent for use in enzyme-linked immunosorbent assays
R G Dietzgen1, R E Snoke, B Babb
1Department of Plant Pathology, Cornell University, Ithaca, New York 14853.
Analytical Biochemistry
|August 1, 1987
Abstract:
A colorimetric reagent, 4-(4'-nitro-2'-methylsulfonylphenylazo)phenyl phosphate (NMPP), has been shown to be an effective substrate of alkaline phosphatase. NMPP and p-nitrophenyl phosphate were applied in comparative studies using enzyme immunoassays for the detection of viral antigens and antiviral antibodies. The new substrate exhibited similar, or even higher, sensitivity than p-nitrophenyl phosphate depending on the substrate concentrations used. Positive and negative reactions were easier to define, even without cumbersome equipment. The enzyme reaction was terminated by the uncompetitive inhibitor, theophylline.