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Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
Published on: October 21, 2016
Fluorescence-detected circular dichroism studies of serum albumins
M P Thomas1, G Patonay, I M Warner
1Department of Chemistry, Emory University, Atlanta, Georgia 30322.
Analytical Biochemistry
|August 1, 1987
Abstract:
Multidimensional fluorescence-detected circular dichroism (FDCD) is used to investigate the binding of bilirubin to four mammalian serum albumins. It is shown that the complexes formed with the albumins have distinctly different FDCD spectra. The effect of the pH-dependent transition from basic to acidic conditions on the complexes is examined. The binding of warfarin to albumin is also presented to demonstrate the general analytical utility of the multidimensional FDCD measurement for biochemical analysis.

